The Effect of Diopatra claparedii Grube, 1878 Aqueous Extract on Chang Liver and Human Primary Glioblastoma (U-87) Cell Lines
DOI:
https://doi.org/10.46754/umtjur.v1i3.79Keywords:
Diopatra claparedii, Ruat Sarung, marine polychaete, cell regeneration, cytotoxicity, Chang Liver cell, Human Primary GlioblastomaAbstract
Due to the distinctive regenerative ability of Diopatra claparedii Grube, 1878, the local marine polychaete has the potential as a cellular growth agent. In this study, the growth effect was investigated in normal cells and cancer cells. Different concentrations (0-100mg/mL) of D. claparedii aqueous extract were tested on Chang Liver (normal cells), and Human Primary Glioblastoma (U-87) (cancer cells) cell lines for 24, 48 and 72 hours. Percentage of cell viability was evaluated by [2-(4, 5-dimethyl-2-thiazolyl)-3, 5-diphenyl-2H tetrazolium bromide] (MTT) assay. The findings suggested that the extract had a proliferative effect on normal cell growth when tested at lower doses (<60 mg/mL) but inhibited normal cells at concentrations >80 mg/mL in all incubation periods. Meanwhile, it showed the cytotoxic effect on cancer cells only after 48h when treated with all concentrations. As demonstrated, the extract could induce normal cell growth without causing abnormal or cancer cells progression at low concentrations after 48h and 72h.
References
Alzheimer's Disease Facts And Figures. (2018). Alzheimer's Association. Retrieved April 16, 2018 from https://www.alz.org/facts/
Bely, A. E. (2006). Distribution of segment regeneration ability in the Annelida. Integrative and Comparative Biology, 46(4), 508–518. https://doi.org/10.1093/icb/icj051
Bely, A. E. (2014). Early Events in Annelid Regeneration: A Cellular Perspective. Integrative and Comparative Biology, 54(4), 688–699. https://doi.org/10.1093/icb/icu109
Calcabrini, C., Catanzaro, E., Bishayee, A., Turrini, E., & Fimognari, C. (2017). Marine Sponge Natural Products with Anticancer Potential: An Updated Review. Marine Drugs, 15(10). https://doi.org/10.3390/md15100310
Cancer Facts & Figures (2018). American Cancer Society. Retrieved September 30, 2018 from https://www.cancer.org/content/dam/cancer-org/research/cancer-facts-and-statistics/annual-cancer-facts-and-figures/2018/cancer-facts-and-figures-2018.pdf
Doll, R. (1995). Chronic and degenerative disease: major causes of morbidity and death. The American Journal of Clinical Nutrition, 62(6 Suppl), 1301S-1305S
Freitas, R., Pires, A., Velez, C., Almeida, Â., Wrona, F. J., Soares, A. M. V. ., & Figueira, E. (2015). The effects of salinity changes on the Polychaete Diopatra neapolitana: Impacts on regenerative capacity and biochemical markers. Aquatic Toxicology, 163, 167–176. https://doi.org/10.1016/j.aquatox.2015.04.006
Glasby, C., Hutchings, P., Fauchald, K., Paxton, H., Rouse, G., Watson, C., & Wilson, R. (2000). Class polychaeta. In: Polychaetes & allies: The southern synthesis. Fauna of australla. Polychaeta, Myzostomida, Pogonophora, Echiura, Sipunculida. 4. 1-296.
Goss, R. J. (2016). Regeneration. Encyclopaedia Brittanica.Retrieved July 15, 2017 from https://www.britannica.com/science/regeneration-biology
Hussain, N. S., Harun, N. A., Mohd Radzi, M. N., Idris, I., & Ismai, W. I. W. (2018). Biosynthesis of silver nanoparticles from marine polychaete Diopatra claparedii Grube, 1878. Jurnal Teknologi, 80(6), 181-187.
Idris, I. & Arshad, A. (2013). Checklist of polychaetous annelids in Malaysia with redescription of two commercially exploited species, Asian Journal of Animal and Veterinary Advances.
Mazliadiyana, M., Nazrun, A. S., & Isa, N. M. (2017). Optimum Dose of Sea Cucumber (Stichopus chloronotus) Extract for Wound Healing. Medicine & Health, 12(1), 83–89. https://doi.org/10.17576/MH.2017.1201.09
Patar, A., Mohsin Syed, S., Jamalullail, S., Jaafar, H., & Abdullah, J. (2012). The effect of water extract of sea cucumber Stichopus variegatus on rat spinal astrocytes cell lines (Vol. 3).
Paxton, H. (2002). Diopatra Audouin and Milne Edwards (Polychaeta: Onuphidae) from Thailand, in Eibye-Jacobsen, D. (ed.) Proceeding of the International Workshop on the Polychaetes of the Andaman Sea June-August 1997. Thailand: Phuket Marine Biological Center, pp. 101–138.
Rajasekaran, A., Sivagnanam, G., Xavier, R. (2008). Nutraceuticals as therapeutic agents: A Review. Research J. Pharm. and Tech. 1(4), 328-340.
Sun, J. M., & Kurtzberg, J. (2015). Chapter 13 - Emerging Uses of Cord Blood in Regenerative Medicine—Neurological Applications. In C. Stavropoulos-Giokas, D. Charron, & C. Navarrete (Eds.), Cord Blood Stem Cells and Regenerative Medicine (pp. 167–177). Boston: Academic Press. https://doi.org/10.1016/B978-0-12-407785-0.00013-X
Thermo Fisher Scientific. (2015). Cell Culture Basics Handbook. United Kingdoms: Author.
Vincent, M. (2014). Business Issues. Principles of Tissue Engineering, 87(4), 1799-1811. http://dx.doi.org/10.1016/B978-0-12-398358-9.00087-2
Wargasetia, T. L., & Widodo. (2017). Mechanisms of cancer cell killing by sea cucumber-derived compounds. Investigational New Drugs, 35(6), 820–826. https://doi.org/10.1007/s10637-017-0505-5
