POPULATION GENETICS OF LONGTAIL TUNA (Thunnus tonggol) (BLEEKER, 1851) FROM EAST MALAYSIA BASED ON MITOCHONDRIAL DNA D-LOOP MARKER

Authors

  • NURUL AZLIANA MOHD YASIN Faculty of Fisheries and Food Science, Universiti Malaysia Terengganu
  • NOORHANI SYAHIDA KASIM Faculty of Fisheries and Food Science, Universiti Malaysia Terengganu
  • TUN NURUL AIMI MAT JAAFAR Faculty of Fisheries and Food Science, Universiti Malaysia Terengganu
  • RUMEAIDA MAT PIAH Faculty of Fisheries and Food Science, Universiti Malaysia Terengganu
  • WAHIDAH MOHD ARSHAAD Marine Fishery Resources Development and Management Department (MFRDMD)
  • SITI AZIZAH MOHD NOR Institute Marine Biotechnology (IMB), Universiti Malaysia Terengganu
  • AHASAN HABIB Department of Fisheries and Marine Science, Noakhli Science and Technology University
  • MIN PAU TAN Institut Biodiversiti Tropika dan Pembangunan Lestari (BIO-D Tropika), Universiti Malaysia Terengganu

DOI:

https://doi.org/10.46754/umtjur.v2i2.146

Keywords:

Thunnus tonggol, mitochondrial DNA D-loop, population structure, genetic diversity, fisheries management

Abstract

Present study investigates the genetic diversity and genetic distribution of the longtail tuna Thunnus tonggol collected from east Malaysia (Borneo states of Sabah and Sarawak) based on mitochondrial DNA D-loop sequence analysis. 58 fish samples were obtained, specifically from Kota Kinabalu, KK (n = 22), Miri, MR (n=20) and Bintulu, BT (n = 17). DNA template was isolated using the salt extraction method. Final length of 404 base pair (bp) D-loop sequences revealed 52 haplotypes that comprise of 77 variable sites (38 of parsimony informative and 39 singleton). A total of 20 haplotypes were found in KK, 19 haplotypes in MR and 16 haplotypes in BT. Molecular diversity indices revealed high haplotype diversity and low nucleotide diversity in all populations; KK (h = 0.9913 ± 0.0165, π = 0.00239 ± 0.0127), MR (h = 0.9942 ± 0.0193, π = 0.0226 ± 0.0121) and BT (h = 0.9926 ± 0.0230, π = 0.0196 ± 0.0171). Population comparison pairwise FST show that KK and BT were significantly genetically differentiated. The result from this study will be beneficial for fisheries management and also to provide information on the population genetics of T. tonggol in East Malaysian waters.

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Additional Files

Published

2020-04-03

How to Cite

MOHD YASIN, N. A. ., KASIM, N. S. ., MAT JAAFAR, T. N. A. ., MAT PIAH, R. ., MOHD ARSHAAD, W. ., MOHD NOR, S. A. ., HABIB, A. ., & TAN , M. P. (2020). POPULATION GENETICS OF LONGTAIL TUNA (Thunnus tonggol) (BLEEKER, 1851) FROM EAST MALAYSIA BASED ON MITOCHONDRIAL DNA D-LOOP MARKER. Universiti Malaysia Terengganu Journal of Undergraduate Research, 2(2), 49–56. https://doi.org/10.46754/umtjur.v2i2.146

Issue

Vol. 2 No. 2 (2020): UMT Journal of Undergraduate Research, April 2020

Section

Articles