Phytochemical study on black pepper berries (Piper nigrum L.)
DOI:
https://doi.org/10.46754/umtjur.v7i1.405Keywords:
Black pepper, piperaceae, flavour compound, secondary metabolite, chromatographyAbstract
Black pepper is one of the well-known spices rich in aromatic and medicinal components, along with substantial levels of several other functional components that have health-promoting properties. The use of black pepper has been recorded in various fields such as traditional medicine, food processing, and the pharmaceutical industry. It is also recognised as an important source of natural antioxidants that have anticarcinogenic properties. In this study, a chemical investigation using black pepper berries (Piper nigrum) was conducted. The purpose of doing that is to determine the chemical component of this species. The sequential maceration extraction methods utilising solvents with different polarities, namely hexane, dichloromethane, and methanol, yielded the corresponding crude extracts. The quantitative phytochemical analysis was performed on the crude extracts. Separation and isolation work was focused on the dichloromethane crude extract using Thin Layer Chromatography (TLC), Column Chromatography (CC), and Preparative Thin Layer Chromatography (PTLC). The outcomes revealed that the berries of P. nigrum contained alkaloid, terpenoid, steroid, and flavonoid metabolites. Two semi-pure compounds have been isolated from the dichloromethane crude extract. The obtained compounds were analysed using Fourier Transform Infrared (FTIR) spectroscopy and Ultraviolet-Visible (UV-Vis) spectrophotometer. Based on the FTIR spectrums and previous studies, these semi-pure compounds, K1A (2) and K3C (3), are expected to be 2, 4-di-tert-butylphenol and ethyl linoleate, respectively. This significant data provides preliminary findings that may lead to further purification and structure determination as a potential source of herbal medicinal compounds.
References
Ahmad, N., Fazal, H., Abbasi, B. H., Rashid, M., Mahmood, T., & Fatima, N. (2010). Efficient regeneration and antioxidant potential in regenerated tissues of Piper nigrum L. Plant Cell, Tissue and Organ Culture (PCTOC), 102(1), 129-134.
Ahmad, R., Ahmad, N., Amir, M., Aljishi, F., Alamer, M. H., Al‐Shaban, H. R., Alsadah, Z. A., Alsultan, B. M., Aldawood, N. A., Chathoth, S., & Almofty, S. A. (2020). Quality variation and standardization of black pepper (Piper nigrum): A comparative geographical evaluation based on instrumental and metabolomics analysis. Biomedical Chromatography, 34(3), Article e4772.
Amir, R. M., Anjum, F. M., Khan, M. I., Khan, M. R., Pasha, I., & Nadeem, M. (2013). Application of Fourier transform infrared (FTIR) spectroscopy for the identification of wheat varieties. Journal of Food Science and Technology, 50(5), 1018-1023.
Anith, K. N., Aswini, S., Varkey, S., Radhakrishnan, N. V., & Nair, D. S. (2018). Root colonization by the Endophytic Fungus Piriformospora indica improves growth, yield and piperine content in black Pepper (Piper nigrum L.). Biocatalysis and Agricultural Biotechnology, 14, 215-220.
Barkat, M. Q., & Mahmood, H. K. (2018). Phytochemical and antioxidant screening of Zingiber officinale, Piper nigrum, Rutag raveolanes and Carum carvi and their effect on gastrointestinal tract activity. Matrix Science Medica, 2(01), 9-13.
Chaveerach, A., Mokkamul, P., Sudmoon, R., & Tanee, T. (2006). Ethnobotany of the Genus Piper (piperaceae) in Thailand. Ethnobotany Research and Applications, 4, Article 223.
Ghodki, B. M., & Goswami, T. K. (2019). Modeling of granular heat transfer in cryogenic grinding system: Black pepper seeds. Process Safety and Environmental Protection, 141, 302-316.
Gorgani, L., Mohammadi, M., Najafpour, G. D., & Nikzad, M. (2016). Piperine—The bioactive compound of black pepper: From isolation to medicinal formulations. Comprehensive Reviews in Food Science and Food Safety, 16(1), 124-140.
Gupta, N., Parashar, P., Mittal, M., Mehra, V., & Khatri, M. (2014). Antibacterial potential of Elletaria cardamomum, Syzygium aromaticum and Piper nigrum, their synergistic effects and phytochemical determination. Journal of Pharmacy Research, 8(8), 1091-1097.
Gupta, M., Gupta, A., & Gupta, S. (2013). In vitro antimicrobial and phytochemical analysis of dichloromethane extracts of Piper nigrum (black pepper). Oriental Journal of Chemistry, 29(2), 777-782.
Harborne, A. B. (1998) Phytochemical methods: A guide to modern techniques of plant analysis (3rd ed.). Springer (India) Private Limited, New Delhi.
Swathy, J. S., Mishra, P., Thomas, J., Mukherjee, A., & Chandrasekaran, N. (2018). Antimicrobial potency of high-energy emulsified black pepper oil nanoemulsion against aquaculture pathogen. Aquaculture, 491, 210 - 220.
Mistry, B. D. (2009). A hand book of spectroscopic data chemistry: UV, IR, PMR, CNMR and Mass Spectroscopy. New Delhi: Oxford Book Co.
Mukherjee P. K. (2002) Quality control of herbal drugs: An approach to evaluation of botanicals (1st ed.). New Delhi: Business Horizons.
Parthasarathy, V., Chempakam, B., & Zachariah, T. J. (2008). Chemistry of Spices. CABI Digital Library. https://doi. org/10.1079/9781845934057.0000
Paulkumar, K., Gnanajobitha, G., Vanaja, M., Rajeshkumar, S., Malarkodi, C., Pandian, K., & Annadurai, G. (2014). Piper NigrumLeaf and Stem assisted green synthesis of silver nanoparticles and evaluation of its antibacterial activity against agricultural plant pathogens. The Scientific World Journal, 2014, 1-9.
Peter, K. V. (2012). Handbook of herbs and spices. Woodhead Pub.
Ravindran, P. N. (2003). Black pepper: Piper nigrum. CRC Press.
Salehi, B., Zakaria, Z. A., Gyawali, R., Ibrahim, S. A., Rajkovic, J., Shinwari, Z. K., Khan, T., Sharifi-Rad, J., Ozleyen, A., Turkdonmez, E., Valussi, M., Tumer, T. B., Monzote Fidalgo, L., Martorell, M., & Setzer, W. N. (2019). Piper species: A comprehensive review on Their phytochemistry, biological activities and applications. Molecules, 24(7), Article 1364.
Srinivasan, K. (2007). Black pepper and its pungent principle-piperine: A review of diverse physiological effects. Critical Reviews in Food Science and Nutrition, 47(8),735-748. https://doi. org/10.1080/10408390601062054
Zahira, A., Thamilmani, K., & Mohamed, R. R. (2016). Phytochemical screening and GC-MS profiling of Piper nigrum L. World Journal of Pharmaceutical Research, 5(5), 798-807.
Zhu, F., Mojel, R., & Li, G. (2018). Physicochemical properties of black pepper (Piper nigrum) starch. Carbohydrate Polymers, 181, 986-993.
Zhuang, J., Li, M., Pu, Y., Ragauskas, A. J., & Yoo, C. G. (2020). Observation of potential contaminants in processed biomass using Fourier Transform infrared spectroscopy.. Applied Sciences, 10(12), Article 43
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