Stability of Lactic Acid Bacteria in Pasteurized Cow’s and Goat’s Milk

Authors

  • Nurfarhana Syed Malik Faculty of Fisheries and Food Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
  • Mohd. Nizam Lani Faculty of Fisheries and Food Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
  • Fauziah Tufail Ahmad Faculty of Fisheries and Food Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

DOI:

https://doi.org/10.46754/umtjur.v1i4.94

Keywords:

Milk, cow, goat, pasteurization, lactic acid bacteria, stability

Abstract

This study was done to determine the effect of pasteurization on the stability of lactic acid bacteria (LAB) and its enzyme in raw and pasteurized cow’s and goat’s milk. The total viable count for plate count of the bacterial growth concentration was higher in both pasteurized cow’s and goat’s milk at 2.48 log CFU/ml. This is followed by raw cow’s milk (1.59 log CFU/ml) and raw goat’s milk (0.65 log CFU/ml). Lactic acid bacteria (LAB) was found to be similar in both raw cow’s and goat’s milk (p>0.05), and pasteurized milk of both animals also contained the same amount of LAB (p>0.05). LAB was still detected in pasteurized milk (p<0.05), indicating the stability of LAB against the pasteurization temperature. Interestingly, based on API ZYM assay kit results, there were nine different enzymes detected in all samples, which were leucinearylamidase, valinearylamidase,cystinearylamidase, trypsin, α-chymotrypsin, naphthol-AS-BI-phosphohydrolase, α-glucosidase, β-glucosidaseand acid phosphatise. The results revealed that different types of lactic acid bacteria were detected in treated and non-treated milk samples produced by different animals, indicating the different stability levels of LAB against pasteurization.

References

Abdul Elrahman, S.M.A., Said, A. M. M., Ahmed, I. E. M, Zeber, E. Z., Owni, O. A. O. & Ahmed, M.K.A. (2013). Effect of Storage Temperature on the Microbiological and Physicochemical Properties of Pasteurized Milk. Annals. Food Science and Technology, 14, 1-7.

Azhari Ali, A. (2011). Isolation and Identification of Lactic Acid Bacteria from Raw Cow Milk in Khartoum State, Sudan. International Journal of Dairy Sciences, 6, 66-71.

Bluma, A., & Ciprovica, I. (2016). Non starter lactic acid bacteria in raw milk thermally treated milk and swiss type cheese.Research for Rural Development, 1, 98-101.

Carminati, D., Tidona, F., Fornasari, M. E., Rossetti, L., Meucci, A., &Giraffa, G. (2014). Biotyping of cultivable lactic acid bacteria isolated from donkey milk. Letters in Applied Microbiology, 59, 299-305.

Claeys, W.L., Cardoena, S., Daubeb, G., Blockc, J., Dewettinckd, K., Dierick, K., Zutter, L., Huyghebaert, A., Imberechts, H., &Thiange, P. (2013). Raw or heated cow milk consumption: Risks and benefits. Food Control, 31, 251-262.

deVrese M., Stagelmann, A., Richter, B., Fenselau, C., Laue, C. &Schrezenmeir, J. (2001). Probiotics-compensation for lactase insufficiency. The American Journal of Clinical Nutrition, 73 (2), 421-429.

Delavenne, E., Mounier, J., Déniel, F., Barbier, G.&Le Blay, G. (2012). Biodiversity of antifungal lactic acid bacteria isolated from raw milk samples from cow, ewe and goat over one-year period. International Journal of Food Microbioogy, 155(3),185–190.

Dumalisile, P. R. Witthuhn, C. &Britz, T. J.(2005). Impact of different pasteurization temperatures on the survival of microbial contaminants isolated from pasteurized milk. International Journal of Dairy Sciences, 58(2), 74-82.

Humble, M. W., King, A. &Phillips, I. (1977). API ZYM: a simple rapid system for the detection of bacterial enzymes. Journal Clinical of Pathology,30(3), 275–277.

Hutkins, R.W. &Nannen, N. L. (1993). "PH Homeostasis in Lactic Acid Bacteria”. Journal of Dairy Science,76(8), 2354-2365.

Khedid, K., Faid, M., Mokhtari, A., Soulaymani, A. & Zinedine, A. (2009). Characterization of lactic acid bacteria isolated from the one humped camel milk produced in Morocco. Microbiological Research,164(1), 81–91.

Quigley,L., O'Sullivan, O., Stanton, C., Beresford, T.P., Ross, R.P., Fitzgerald, G.F. &Cotter, P.D. (2013). The complex microbiota of raw milk, FEMS Microbiology 37(5), 664–698.

Patel, A., Shah, N. &Prajapati, J.B. (2013). Biosynthesis of vitamins and enzymes in fermented foods by lactic acid bacteria and related genera. Journal of Crop Science and Technology. 5(2), 85-91.

Samarzija, D., Zamberlin, S. &Pogacic, T. (2012). Psychotropic bacteria and milk and dairy products quality, Mljekarstvo,62 (2), 77-95.

Schillinger, U. & Villarreal, J.V. (2010). Inhibition of PenicilliumNordicum in MRS Medium by Lactic Acid Bacteria Isolated from Foods, Food Control, 21, 107-111.

Sengun, I.Y., Nielsen, D.S., Karapinar, M. &Jakobsen, M. (2009). Identification of Lactic Acid Bacteria Isolated from Tarhana, a Traditional Turkish Fermented Food. International Journal of Food Microbiology, 135, 105-111.

Stoyanovski, S., Gacovski, Z., Antonova-Nikolova, S., Kirilov, N., Ivanova, I., Tenev, T. &

Had-Jinesheva, V.(2013). API ZYM enzymatic profile of lactic acid bacteria isolated from traditional Bulgarian meat product “Lu-kanka”. Bulgarian Journal of Agricultural Science, 2 (19), 86–89.

Tamine, A.Y., &Robinson, R.K. (1999).Yoghurt: Science and Technology Woodhead Publication.

Tasci, F. (2011). Microbiological and Chemical Propertiesof Raw Milk Consumed in Budur. Journal of Veterinary Advances, 10 (5), 635-641.

Tesfay, T., Kebede, A. &Seifu, E. (2015).Physico Chemical Properties of Cow Milk Produced and Marketed in Dire Dawa Town, Eastern Ethiopia. Food Science and Quality Management, 42, 56-61.

Teshome, G. (2015). Review on lactic acid bacteria function in milk fermentation and preservation. African Journal of Food Science, 9(4), 170–175.

Wassie,M. &Wassie, T. (2016). Isolation and Identification of Lactic Acic Bacteria from Raw Cow Milk. InternationalJournal of Advanced Research in Biological Science, 3(8), 44-49.

Wedajo B. (2015). Lactic Acid Bacteria: Benefits, Selection Criteria and Probiotic Potential in Fermented Food. Journal of Problem Health, 3, 129.

Widyastuti, Y. &Febrisiantosa, R, A. (2014). The Role of Lactic Acid Bacteria in Milk Fermentation. Food and Nutrition Science, 5, 435-442.

Additional Files

Published

2019-10-31

How to Cite

Nurfarhana Syed Malik, Mohd. Nizam Lani, & Fauziah Tufail Ahmad. (2019). Stability of Lactic Acid Bacteria in Pasteurized Cow’s and Goat’s Milk. Universiti Malaysia Terengganu Journal of Undergraduate Research, 1(4), 77–82. https://doi.org/10.46754/umtjur.v1i4.94