18S DNA BARCODING AND PHYLOGENETIC ANALYSIS OF MARINE MACROALGAE FROM UMT BEACH, KUALA TERENGGANU, MALAYSIA
DOI:
https://doi.org/10.46754/umtjur.v7i2.583Keywords:
Cladophora sp., DNA Barcoding, Macroalgae, Pyropia sp., 18S PrimerAbstract
Macroalgae play a crucial role in maintaining marine ecosystems and assimilating carbon dioxide through photosynthesis. Deoxyribonucleic acid (DNA) barcoding is essential for accurately identifying marine macroalgae, particularly in Southeast Asian waters. Accurate species identification is crucial for marine resource sustainability and biodiversity conservation. This study identifies marine macroalgae and their phylogenetic relations at the University Malaysia Terengganu (UMT) Beach. The samples were cleaned, morphologically identified, and the DNA was extracted using the Cetyltrimethylammonium Bromide (CTAB) method. The DNA template was then amplified using an 18S primer. The resulting Polymerase Chain Reaction (PCR) products were forwarded to Apical Scientific Sdn. Bhd. for sequencing. Subsequently, the DNA sequences were imported into the MEGA11 software and used to construct a phylogenetic tree. The research findings conclude that UMT Beach, Kuala Terengganu, Malaysia, reveals Cladophora sp. and Pyropia sp. macroalgae species with distant phylogenetic relationships, as determined using DNA barcoding protocols.ding protocols.
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