Effect of Sucrose Concentration on Proliferation of Bucephalandra Sp. ‘Red-Mini’ Cultures
DOI:
https://doi.org/10.46754/umtjur.v7i1.492Keywords:
Aquatic plant, reducing sugar, plantlets, shoot tips, culture mediumAbstract
Bucephalandra sp. ‘Red Mini’ is an aquatic flowering plant endemic to Borneo Island. The plant is popular among aquarists due to its magnificent appearance underwater submergence. Its vegetative propagation is relatively slow. Thus, applying the tissue culture technique could increase the production of diseased-free plantlets in large quantities within a short period. To date, limited reports are available on the effects of sucrose on the multiplication of Bucephalandra sp. ‘Red Mini’. Therefore, this study investigated the effects of sucrose concentration on the number of shoot tips and reduced sugar content of Bucephalandra sp. ‘Red Mini’ cultures. The plantlets were cultured in MS medium containing various sucrose concentrations 1%, 3%, or 5% (w/v) added with 0 or 0.5 mg/L of Thidiazuron (TDZ), respectively. The newly formed shoot tips were recorded every two weeks intervals for 10 weeks and subsequently measured for the reducing sugar content. Results revealed that the new shoot tip number produced and the reduced sugar content were influenced by sucrose concentration and the presence of TDZ in the media. The highest shoot tip was recorded in the treatment medium containing 5.0% (w/v) sucrose added with 0.5 mg/L TDZ. In this medium, the shoot tip produced and reducing sugar content were 23.3 ± 4.0 shoot tips/culture and 0.35 ± 0.01 mM/culture, respectively. The finding suggested that sucrose concentration and TDZ synergistically regulate the formation of new shoot tips of Bucephalandra sp. ‘Red Mini’ and the reduced sugar content in the explants.
References
Barpete, S., Özcan, S. F., Aasim, M., & Özcan, S. (2015). In vitro high-frequency regeneration through apical shoot proliferation of Hemianthus callitrichoides ‘Cuba’ ‐ a multipurpose ornamental aquatic plant. Turkish Journal of Biology, 39, 493- 500. https://doi.org/10.3906/biy-1411-71 DOI: https://doi.org/10.3906/biy-1411-71
Boyce, P., Wong, S. Y., Ting, A., Low, S., Low, S., Ng, K., & Ooi, I. (2010). The Araceae of Borneo genera. Aroideana, 33, 3-74.
Braun, D. M. (2022). Phloem loading and unloading of sucrose: What a long, strange trip from source to sink. Annual Review of Plant Biology, 73(1), 553-584. https:// doi.org/10.1146/annurev-arplant-070721 -083240 DOI: https://doi.org/10.1146/annurev-arplant-070721-083240
Del Rosario Cárdenas-Aquino, M., Camas- Reyes, A., Valencia-Lozano, E., López- Sánchez, L., Martínez-Antonio, A., & Cabrera-Ponce, J. L. (2023). The cytokinins BAP and 2-iP modulate different molecular mechanisms on shoot proliferation and root development in lemongrass (Cymbopogon citratus). Plants, 12(20), Article 3637. https://doi.org/10.3390/plants12203637 DOI: https://doi.org/10.3390/plants12203637
Chandrasekhara R. M., Bramhachari, P. V., & Sri Rama Murthy, K. (2015). Optimised plant tissue culture protocol for in vitro morphogenesis of an endangered medicinal herb Ceropegia ensifolia Bedd. Tropical and Subtropical Agroecosystems, 18(1), 95- 101. DOI: https://doi.org/10.56369/tsaes.2099
Chen, J. & Wei, X. (2018). Thidiazuron in micropropagation of Aroid plants. In Ahmad, N., Faisal, M. (Eds.). Thidiazuron: From urea derivative to plant growth regulator. Springer, Singapore. https://doi. org/10.1007/978-981-10-8004-3 DOI: https://doi.org/10.1007/978-981-10-8004-3_4
Du, H. M., Tang, D. M., & Huang, D. F. (2006). ‘Fragrant taro’ [Colocasia esculenta (L.) Schott var. antiquorum] micropropagation using thidiazuron and benzylaminopurine. The Journal of Horticultural Science and Biotechnology, 81(3), 379-384. https://doi. org/10.1080/14620316.2006.11512076 DOI: https://doi.org/10.1080/14620316.2006.11512076
Fujita, H. & Kawaguchi, M. (2011). Strategy for shoot meristem proliferation in plants. Plant Signalling and Behaviours, 6(911), 1851-1854. DOI: https://doi.org/10.4161/psb.6.11.17656
Fitriani, H., Aryaningrum, P. D. W. I., & Hartati, N. S. R. I. (2016). The proliferation of embryogenic callus of Satoimo taro (Colocasia esculenta var. antiquorum) in culture media with various levels of sucrose and gelling agent. Nusantara Bioscience, 8(1), 316-320. DOI: https://doi.org/10.13057/nusbiosci/n080230
Gaikwad, A. V., Singh, S. K., & Gilhotra, R. (2017). Plant tissue culture - A review. Journal of Pharmaceutical Research and Education, 2(1), 217-220.
Gamborg, O. L., Miller, R. A., & Ojima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50(1), 151-158. DOI: https://doi.org/10.1016/0014-4827(68)90403-5
Gaveau, D. L. A., Sloan, S., Molidena, E., Yaen, H., Sheil, D., Abram, N. K., Ancrenaz, M., Nasi, R., Quinones, M., Wielaard, N., & Meijaard, E. (2014). Four decades of forest persistence, clearance and logging on Borneo. PLOS ONE, 9(7), e101654. https:// doi.org/10.1371/journal.pone.0101654 DOI: https://doi.org/10.1371/journal.pone.0101654
Granot, D., David-Schwartz, R., & Kelly, G. (2013). Hexose kinases and their role in sugar-sensing and plant development. Frontiers in Plant Science, 4, 1-17. https:// doi.org/10.3389/fpls.2013.00044 DOI: https://doi.org/10.3389/fpls.2013.00044
Guo, B., Bilal, H. A., Amir, Z., Xu, L. L., & Wei, Y. H. (2011). Thidiazuron: A multidimensional plant growth regulator. African Journal of Biotechnology, 10(45), 8984-9000. https://doi.org/10.5897/AJB11. 636 DOI: https://doi.org/10.5897/AJB11.636
Hatanaka, C., & Kobara Y. (1980) Determination of glucose by a modification of Somogyi- Nelson method. Agriculture and Biological Chemistry, 44(12), 2943-2949. DOI: https://doi.org/10.1080/00021369.1980.10864408
Jiang, Z., Wang, M., Nicolas, M., Ogé, L., Pérez-Garcia, M., Crespel, L., Li, G., Ding, Y., Gourrierec, J. L., Grappin, P., & Sakr, S. (2022). Glucose-6-Phosphate dehydrogenases: the hidden players of plant physiology. International Journal of Molecular Sciences, 23(24), Article 16128. https://doi.org/10.3390/ijms232416128 DOI: https://doi.org/10.3390/ijms232416128
Jo, E., Tewari, R. K., Hahn, E., & Paek, K. (2008). In vitro sucrose concentration affects growth and acclimatisation of Alocasia amazonica plantlets. Plant Cell Tissue and Organ Culture, 96(3), 307-315. https://doi.org/10.1007/s11240-008-9488-4 DOI: https://doi.org/10.1007/s11240-008-9488-4
Karataş, M., & Aasim, M. (2014). Efficient in vitro regeneration of medicinal aquatic plant water hyssop (Bacopa monnieri L. PENNELL), Pakistan Journal of Agricultural Science, 51(3), 667-672.
Lemoine, R. (2000). Sucrose transporters in plants: Update on function and structure. Biochimica et Biophysica Acta - Biomembranes, 1465(1-2), 246-262. https:// doi.org/10.1016/S0005-2736(00)00142-5 DOI: https://doi.org/10.1016/S0005-2736(00)00142-5
Li, J., He, C., Liu, S., Guo, Y., Zhang, Y., Zhang, L., Zhou, X., Xu, D., Luo, X., Liu, H., Yang, X., Wang, Y., Shi, J., Yang, B., Wang, J., Wang, P., Deng, X., & Sun, C. (2024). Research progress and application strategies of sugar transport mechanisms in rice. Frontiers in Plant Science, 15, Article 1454615. https://doi.org/10.3389/ fpls.2024.1454615 DOI: https://doi.org/10.3389/fpls.2024.1454615
Long, J. A., Moan, E. I., Medford, J. I., & Barton, M. K. (1996). A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis. Nature, 379, 66-69. DOI: https://doi.org/10.1038/379066a0
Lopes, F. L., Formosa-Jordan, P., Malivert, A., Margalha, L., Confraria, A., Feil, R., Lunn, J. E., Jönsson, H., Landrein, B., & Baena- González, E. (2023). Sugar signaling modulates SHOOT MERISTEMLESS expression and meristem function in Arabidopsis. bioRxiv (Cold Spring Harbor Laboratory). https://doi. org/10.1101/2023.01.08.522175 DOI: https://doi.org/10.1101/2023.01.08.522175
Madsen, J. D., & Wersal, R. M. (2017). A review of aquatic plant monitoring and assessment methods. Journal of Aquatic Plant Management, 55, 1-12.
Martins, J. P. R., Pasqual, M., Martins, A. D. & Ribeira, S. F. (2015). Effects of salts and sucrose concentrations on in vitro propagation of Billbergia zebrina (Herbert) Lindley (Bromeliaceae). Australian Journal of Crop Science, 9(1), 85-91
Mashhor, M., Boyce, P. C., Othman, A. S., & Sulaiman, B. (2012). The Araceae of Peninsular Malaysia. Pulau Pinang: Penerbit Universiti Sains Malaysia.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497 DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Murthy, B. N. S., Murch, S. J., & Saxena, P. K. (1998). Thidiazuron: A potent regulator of in vitro plant morphogenesis. In Vitro Cellular and Developmental Biology - Plant, 34(4), 267-275. https://doi.org/10.1007/ BF02822732 DOI: https://doi.org/10.1007/BF02822732
Norhanizan, S., & Aziz, A. (2018). Thidiazuron amends the organ development of endangered aquatic plantCryptocoryne elliptica Hook. F. IOP Conference Series Materials Science and Engineering, 440, Article 012046. https://doi. org/10.1088/1757-899x/440/1/012046 DOI: https://doi.org/10.1088/1757-899X/440/1/012046
O’Hare, M. T., Aguiar, F. C., Asaeda, T., Bakker, E. S., Chambers, P. A., Clayton, J. S., Elger, A., Ferreira, T. M., Gross, E. M., Gunn, I. D. M., Gurnell, A. M., Hellsten, S., Hofstra, D. E., Li, W., Mohr, S., Puijalon, S., Szoszkiewicz, K., Willby, N. J., & Wood, K. A. (2017). Plants in aquatic ecosystems: Current trends and future directions. Hydrobiologia, 812(1), 1-11. https://doi. org/10.1007/s10750-017-3190-7 DOI: https://doi.org/10.1007/s10750-017-3190-7
Petruzzella, A., Manschot, J., Van Leeuwen, C. H. A., Grutters, B. M. C., & Bakker, E. S. (2018). Mechanisms of invasion resistance of aquatic plant communities. Frontiers in Plant Science, 9. https://doi.org/10.3389/ fpls.2018.00134 DOI: https://doi.org/10.3389/fpls.2018.00134
Sama, A. E., Hughes, H. G., Abbas, M. S., & Shahba, M. A. (2012). An efficient in-vitro propagation protocol of cocoyam Xanthosoma sagittifolium (L) Schott. The Scientific World Journal, Article ID 346595. https://doi.org/10.1100/2012/346595 DOI: https://doi.org/10.1100/2012/346595
Sotiropoulos, T. E., Molassiotis, A. N., Mouhtaridou, G. I., Papadakis, I., Dimassi, K. N., Therios, I. N., & Diamantidis, G. (2006). Sucrose and sorbitol effects on shoot growth and proliferation in vitro, nutritional status and peroxidase and catalase isoenzymes of M 9 and MM 106 apple (Malus domestica Borkh.) rootstocks. European Journal of Horticultural Science, 71(3), 114-119. DOI: https://doi.org/10.1079/ejhs.2006/338654
Sumaryono, Muslihatin, W., & Ratnadewi, D. (2012). Effect of carbohydrate source on growth and performance of in vitro sago palm (Metroxylon sagu Rottb.) plantlets. HAYATI Journal of Biosciences, 19(2), 88- 92. https://doi.org/10.4308/hjb.19.2.88 DOI: https://doi.org/10.4308/hjb.19.2.88
Wong, S. Y., & Boyce, P. C. (2016). Studies on Schismatoglottideae (Araceae) of Borneo LVII: Bucephalandra filiformis – a new species from Maligan, Sarawak, Malaysian Borneo. Aroideana, 39(2), 56-60. https:// ir.unimas.my/id/eprint/14341/
Wang, L., & Ruan, Y. (2013). Regulation of cell division and expansion by sugar and auxin signaling. Frontiers in Plant Science, 4, Article 163. https://doi.org/10.3389/ fpls.2013.00163 DOI: https://doi.org/10.3389/fpls.2013.00163
Additional Files
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Universiti Malaysia Terengganu Journal of Undergraduate Research
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
