IMPACT OF pH, TEMPERATURE AND GAMMA RADIATION ON G6PD ACTIVITY AND ERYTHROCYTE MORPHOLOGY IN G6PD DEFICIENCY
DOI:
https://doi.org/10.46754/umtjur.2026.04.001Keywords:
Radiation effect, G6PD, erythrocytes, pH, enzymes.Abstract
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a genetic condition that primarily affects erythrocytes. G6PD is an enzyme predominantly found in erythrocytes, where it catalyses the oxidation of glucose-6-phosphate in glucose metabolism. Erythrocytes are blood cells produced by stem cells in the bone marrow. Previous research on G6PD deficiency has mainly focused on affected patients. Therefore, the objectives of this study are to optimise the effects of pH and temperature on G6PD enzyme activity, investigate the impact of G6PD deficiency on erythrocytes, and examine the morphology of both normal and G6PD-deficient erythrocytes after exposure to gamma radiation. For the methodology, the dilution method was employed to identify the optimal parameters for G6PD activity. Erythrocytes were obtained from both G6PD-deficient patients and healthy individuals. To study the reactions, substances such as uncoated aspirin and broad bean solutions were added to the erythrocytes. The erythrocytes’ morphology was then examined after exposure to gamma radiation from Cesium-137 for a week. After one week, the samples were observed under a Nikon Eclipse LV/UDM microscope. The results from this irradiation serve as evidence of the effects of G6PD deficiency on erythrocytes and highlight substances that may contribute to the deficiency.
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