Radiographic Imaging Application in Capturing Nest Escaping Movement by Sea Turtle Hatchlings
DOI:
https://doi.org/10.46754/umtjur.v7i1.476Keywords:
Sustainability, aggregation behaviour, social facilitation, endangered species, sea turtlesAbstract
Nest escaping is a vital process that takes up a significant amount of energy and time during the early life of sea turtle hatchlings. Previous studies have shown how hatchlings benefit from this aggregation behaviour while digging upward in the nested column. However, there is a lack of information on the relationship between the dimensions of nest escaping movement formation. This study explores the potential of radiographic imaging in capturing the dimension of digging formation to relate its potential with the energy conservation mechanism. This article describes the challenges and prospects of capturing hatchlings’ movement while digging up and escaping their underground nest via radiographic imaging (X-ray). Several trials have been conducted to seek the most suitable approach to be applied as a standard method for future studies. We developed an open-respirometry chamber with a supply of oxygen for egg incubation according to preferred clutch sizes to meet one of the objectives, which is to determine the effect of clutch sizes on the dimension of digging formation. Exposure usage of 125 kV, 40.0 mAs, and 62.5 ms were determined to produce significant X-ray images. Additionally, iron flux has been determined to be useful in measuring hatchlings’ digging progress, as it has the potential to enhance our image quality and observation. We were able to observe the position of the hatchlings with such development. Nevertheless, we do not know the accurate dimension of digging movement formation as modelled by the hatchlings.
References
Ackerman, R. A. (1997). The nest environment and the embryonic development of sea turtles. In Lutz, P. L., & Musick, J. A. (Eds.), The biology of sea turtles (Vol. I, pp. 83-106). CRC Press.
Baker, S. R. (2010). The practical approach to radiology. Springer.
Carr, B. A., & Hirth, H. (1960). Social facilitation in green turtle siblings. Animal Behaviour, 9(1-2), 68-70. https://doi. org/10.1016/0003-3472(61)90051-3 DOI: https://doi.org/10.1016/0003-3472(61)90051-3
Couzin, I. D., Krause, J., James, R., Ruxton, G. D., & Franks, N. R. (2002). Collective memory and spatial sorting in animal groups. Journal of Theoretical Biology, 218(1), 1-11. https://doi.org/10.1006/ jtbi.2002.3065 DOI: https://doi.org/10.1006/jtbi.2002.3065
Delm, M. (1990). Vigilance for predators: Detection and dilution effects. Behavioral Ecology and Sociobiology, 26, 337- 342. https://doi.org/10.1007/BF00171099 DOI: https://doi.org/10.1007/BF00171099
Folk, R. L., & Ward, W. C. (1957) A study in the significance of grain-size parameters. Journal of Sedimentary Petrology, 27, 3-26. DOI: https://doi.org/10.1306/74D70646-2B21-11D7-8648000102C1865D
Harrington, D. (2004). 91 - Imaging Devices. In J. F. Dyro (Ed.), Biomedical Engineering, Clinical Engineering Handbook (pp. 392- 400). Academic Press. DOI: https://doi.org/10.1016/B978-012226570-9/50100-9
Kei Ma, W., Hogg, P., & Norton, S. (2014). Effects of kilovoltage, milliampere seconds, and focal spot size on image quality. Radiologic Technology, 85(5), 479-485.
Knoll, G. F. (2010). Radiation detection and measurement (3rd ed., pp. 29-70). John Wiley & Sons.
Levin, S. A. (1997). Conceptual and methodological issues in the modeling of biological aggregations. In J. K. Parrish & W. M. Hamner (Eds.). Animal Groups in Three Dimensions (pp. 247-256). Cambridge University Press, Cambridge, U.K. DOI: https://doi.org/10.1017/CBO9780511601156.016
Lissaman, P. B. S., & Shollenberger, C. A. (1970). Formation flight of birds. Science, 168(3934), 1003-1005. https://doi. org/10.1126/science.168.3934.1003 DOI: https://doi.org/10.1126/science.168.3934.1003
Miller, J. D., Limpus, C. J., & Godfrey, M. H. (2003). Nest site selection, oviposition, eggs, development, hatching, and emergence of loggerhead turtles. In Lutz, P. L., Musick, J. A., & Wyneken, J. (Eds.), The biology of sea turtles (Vol. II, pp. 125-143). CRC Press.
Najwa-Sawawi, S., Azman, N. M., Rusli, M. U., Ahmad, A., Fahmi-Ahmad, M., & Fadzly, N. (2021). How deep is deep enough? Analysis of sea turtle eggs nest relocation procedure at Chagar Hutang Turtle Sanctuary. Saudi Journal of Biological Sciences, 28(9), 5053-5060. https://doi. org/10.1016/j.sjbs.2021.05.021 DOI: https://doi.org/10.1016/j.sjbs.2021.05.021
Nishizawa, H., Hashimoto, Y., Rusli, M. U., Ichikawa, K., & Joseph, J. (2021). Sensing underground activity: Diel digging activity pattern during nest escape by sea turtle hatchlings. Animal Behaviour, 177, 1-8. https://doi.org/10.1016/j. anbehav.2021.04.013 DOI: https://doi.org/10.1016/j.anbehav.2021.04.013
Packard, G. C., Miller, K., & Packard, M. J. (1993). Environmentally induced variation in body size of turtles hatching in natural nests. Oecologia, 93(3), 445-448. https:// doi.org/10.1007/bf00317890 DOI: https://doi.org/10.1007/BF00317890
Rehani, M. M. (2013). Challenges in radiation Protection of Patients for the 21st century. American Journal of Roentgenology, 200(4), 762-764. https://doi.org/10.2214/ ajr.12.10244 DOI: https://doi.org/10.2214/AJR.12.10244
Rusli, M. U., & Booth, D. T. (2016). Bigger clutch sizes save offspring energy during nest escapes. Behavioral Ecology and Sociobiology, 70(4), 607-616. https://doi. org/10.1007/s00265-016-2079-1 DOI: https://doi.org/10.1007/s00265-016-2079-1
Rusli, M. U., Booth, D. T., & Joseph, J. (2016). Synchronous activity lowers the energetic cost of nest escape for sea turtle hatchlings. Journal of Experimental Biology, 219(10), 1505-1513. https://doi.org/10.1242/ jeb.134742 DOI: https://doi.org/10.1242/jeb.134742
Shafiei, S., Hasanzadeh, H., & Shafiei, S. (2012). A simple calculation method for determination of equivalent square field. Journal of Medical Physics, 37(2), 107-111. https://doi.org/10.4103/0971-6203.94746 DOI: https://doi.org/10.4103/0971-6203.94746
Smith, M. J., & Taylor, J. (2009). Radiographic techniques and applications in marine biology. Journal of Marine Biology and Ecology, 27(3), 445-458.
Spotila, J. A. (2004). Sea turtles: A complete guide to their biology, behavior, and conservation. Baltimore: Johns Hopkins University Press. DOI: https://doi.org/10.56021/9780801880070
Weimerskirch, H., Martin, J., Clerquin, Y., Alexandre, P., & Jiraskova, S. (2001). Energy saving in flight formation. Nature, 413(6857), 697-698. https://doi. org/10.1038/35099670 DOI: https://doi.org/10.1038/35099670
Additional Files
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Universiti Malaysia Terengganu Journal of Undergraduate Research

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

