PHYTOCHEMICAL SCREENING AND EVALUATION OF THE ENZYMATIC ANTIOXIDANT ACTIVITIES OF SELECTED Mangifera spp. LEAVES (ANACARDIACEAE)
DOI:
https://doi.org/10.46754/umtjur.v2i4.173Keywords:
Phytochemical screening, catalase (CAT), guaiacol peroxidase (gPOD), Mangifera sppAbstract
A study was conducted on the chemical and biological properties of three different species of Mangifera i.e. Mangifera pajang, M. indica L., and M. kemanga leaves obtained from Pitas, Sabah. The aims of this study were to determine the presence of secondary metabolites as well as the antioxidative activities especially the catalase (CAT) and guiacol peroxidase specific activities (gPOD) in the leaves part of these three species. The extraction of these samples was carried out using three different polarities of solvents: hexane, ethyl acetate, and methanol. The total percentage of the crude extract of is 7.30% for M. pajang, 12.87% for M. indica and 7.98% for M. kemanga. Phytochemical screening was performed with various tests for each of the crude extracts. The results showed that these three species gave positive results for alkaloids, saponins, flavonoids, phenols, carbohydrates, phytosterols, and tannins metabolites. Based on the tests, CAT specific activities were significantly higher in the leaves of M. pajang with 4.35 ± 1.18 units/mg protein compared to M. indica L. and M. kemanga. The guaiacol peroxidase (gPOD) specific activities showed that M. indica L. has the highest activity with the value of 0.0047 ± 0.0004 units/mg protein.
References
Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D., & Lightfoot, D. (2017). Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants, 6(4), 42.
Azarmehr, B., Karimi, F., Taghizadeh, M., & Seyed Latif, M. G. (2013). Secondary Metabolite Contents and Antioxidant Enzyme Activities of Cichorium intybus Hairy Roots in Response to Zinc. Journal of Medicinal Plants and By-products, 2,131-138.
Badmus, J.A., Adedosu, T.O., Fatoki, J.O., Adegbite, V.A., Adaramoye, O.A. & O.A. Odunola. (2011). Lipid peroxidation inhibition and antiradical activities of some leaf fractions of Mangifera indica lipid peroxidation inhibition and antiradical activities. Acta Poloniae Pharmaceutica Drug Research, 68 (1) (2011), pp. 23-29.
Bhatia, V. K., Ramanathan, J. D., & Seshadri, T.R. (1967). Constitution of mangiferin. Tetrahedron, 23, 1363- 1368.
Bompard, J. M., & Schnell, R.J. (1997). Taxonomy and Systematics. In: R.E. Litz (ed.). The Mango: Botany, Production and Uses. CAB Intl., Wallingford, U.K. pp 21-47.
Desai, P. D., Ganguly, A. K., Govindachari, T. R., Joshi, B. S., Kamat, V. N., Manmade, A. H., Mohamed, P. A., Nagle, S. K., Nayak, R. H., Saksena, A. K., Sathe, S. S., & Vishwanathan, N. (1966). Chemical investigation of some Indian plants: Part II. Indian Journal of Chemistry, 4, 457-549.
Dias, D. A., Urban, S., & Roessner, U. (2012). A historical overview of natural products in drug discovery. Metabolites, 2(4), 303 – 336.
El Ansari, M. A., Rajadurai, S., & Nayudamma, Y. (1967). Studies on the polyphenols of Mangifera indica bark. Leather Science, 14, 247-251.
El Ansari, M. A., Reddy, K. K., Sastry, K. N. S., & Nayudamma. Y. (1971). Dicotyledonae, anacardiaceae polyphenols of Mangifera indica. Phytochemistry, 10, 2239-2241.
Hafsi, C., Romero Puertas M. C., Rio, L. A., Abdelly, C. H., & Sandalio L. M. (2011). Antioxidative response of Hordeum maritimum L. to potassium deficiency. Acta Physiol Plantar, 33, 193-202.
Kanwal, Q., Hussain, I., Siddiqui, H.L. & Javaid, A. (2010). Antifungal activity of flavonoids isolated from mango (Mangifera indica L.) leaves. Natural Product Research, 24:20, 1907-1914.
Nigam, S. K., & Mitra, C.R. (1964). Constituents of mango (Mangifera indica) roots. Indian Journal of Chemistry, 2, 378-379.
Richardson, P. M. (1984). The taxonomic significance of xanthones in ferns. Biochemical Systematics and Ecology, 12, 1-6.
Ross, I. A. (1999). Medicinal Plants of the world, chemical constituents, traditional and modern medicinal uses. Humana Press, Totowa, 197-205.
Scartezzini, P., & Speroni, E. (2000). Review on some plants of Indian traditional medicine with antioxidant activity. Journal of Ethnopharmacology, 71, 23-43.
Severi, J.A., Lima, Z.P., Kushima, H.; Monteiro Souza Brito, A.R., Campaner dos Santos, L., Vilegas, W. & Hiruma- Lima, C.A. (2009). Polyphenols with Antiulcerogenic Action from Aqueous Decoction of Mango Leaves (Mangifera indica L.). Molecules, 14, 1098-1110.
Yoshimi, N., Matsunaga, K., Katayama, M., Yamada, Y., Kuno, T., Qiao, Z., Hara, A., Yamahara, J., & Mori, H. (2001). The inhibitory effects of mangiferin, a naturally occuring glucosylxanthone, in bowel carcinogenesis of male F344 rats. Cancer Letters, 163, 163-170.
