PHYSICOCHEMICAL PROPERTIES OF UNDULATED SURF CLAM (Paphia undulata) HYDROLYSATE AS AFFECTED BY DEGREE OF HYDROLYSIS
DOI:
https://doi.org/10.46754/umtjur.v2i3.157Keywords:
undulated surf clam, protein hydrolysate, degree of hydrolysis, physicochemical properties, Paphia undulataAbstract
This study aimed to determine the physicochemical properties of undulated surf clam (Paphia undulata) hydrolysate as affected by the degree of hydrolysis (DH). Three levels of DH of undulated surf clam hydrolysate were prepared which were DH 36.57% (without any enzymatic hydrolysis), DH 58.25% (0.5% Alcalase®; 5 min; pH 7.5; 60ºC) and DH 91.26% (1% Alcalase®; 30 min; pH 7.5; 60ºC). After protein hydrolysis, the undulated surf clam hydrolysates were centrifuged, and their supernatants were freeze-dried. This study found that the protein hydrolysate with lower DH (DH 36.57%) gave lower protein content and higher ash and fat contents compared to other samples (DH 58.25% and DH 91.26%). However, the carbohydrate content is similar in all samples (16.56-20.04%). This study also found that foaming properties (29.43-67.50%), emulsifying capacity (11.94-110.52%) and peptide solubility (57.61-94.08%) were affected by the DH. As DH increased, the emulsifying capacity decreased, while peptide solubility increased. While the foaming capacity increased with increasing DH until it reached a maximum value and level off afterwards. For colour parameters, although there were differences between L*, a* and b* values for all three samples, a fluctuating pattern was noted with DH. DH also did not affect the water-holding and oil-holding capacity of undulated surf clam hydrolysate. This study shows that certain physicochemical properties of undulated surf clam hydrolysate can be tailored by adjusting the degree of hydrolysis.
References
Abdul-Hamid, A., Jamiah, B., & Bee, G. H. (2002). Nutritional quality of spray dried protein hydrolysate from Black Tilapia. Food Chemistry, 78, 69-74.
Adler-Nissen, J. (1986). Enzymatic hydrolysis of food proteins. New York: Elsevier Applied Science Publishers Ltd.
Amiza, M. A., & Masitah, M. (2012). Optimization of enzymatic hydrolysis of blood cockle (Anadara granosa) using Alcalase. Borneo Science, 31, 1-10.
Amiza, M. A., Kong. Y. L., & Faazaz, A. L. (2012). Effects of degree of hydrolysis on physicochemical properties of cobia (Rachycentron canadum) frame hydrolysate. International Food Research Journal, 19(1), 199-206.
Amiza, M. A., Ow, Y. W., & Faazaz, A. L. (2013). Effect of degree of hydrolysis on physicochemical properties of silver catfish (Pangasuis sp) frame hydrolysate. International Food Research Journal, 20(3), 1255-1262.
AOAC. (2000). AOAC Official Methods of Analysis, 17th edition. Washington: Association of Official Analytical Chemist.
Benjakul, S., & Morrissey, M. T. (1997). Protein hydrolysate from visceral waste protein of Catla (Catla catla): Optimization of hydrolysis conditions for a commercials neutral protease. Bioresource Technology, 99, 4105-4111.
Chalamiaiah, M., Kumar, B. D., Hemalatha, R., & Jyothimayi, T. (2012). Fish protein hydrolysates: Proximate composition, amino acid composition, antioxidant activities and applications: A review. Food Chemistry, 135(4), 3020-3038.
Chanrachkij, I. (2013). Monitoring the Undulated Surf Clam Resources of Thailand for Sustainable Fisheries Management. Fish for the People, 11(3), 33-44.
Chau, C. F., & Cheung, P. C. K. (1998). Functional properties of flours prepared from three Chinese indigenous legume seeds. Food Chemistry, 61(4), 429–433.
Choi, Y. J., Hur, S., Choi, B.-D., Konno, K., & Park, J. W. (2009). Enzymatic Hydrolysis of Recovered Protein from Frozen Small Croaker and Functional Properties of Its Hydrolysates. Journal of Food Science, 74(1), 17–24.
Clemente, A., Vioque, J., Sánchez-Vioque, R., Pedroche, J., Bautista, J., & Millán, F. (2000). Factors affecting the in vitro protein digestibility of chickpea albumins. Journal Science Food and Agriculture, 80, 79-84.
Dickinson, E. (1997). Properties of Emulsions Stabilized with Milk Proteins: Overview of Some Recent Developments. Journal of Dairy Science, 80(10), 2607–2619.
Diniz, F. M. & Martin, A. M. (1997). Effect of the extent of enzymatic hydrolysis on the functional properties of shark protein hydrolysate. Lebensmittel-Wissenschaft and Technologies, 30, 266-272.
Don, B. L. S., Pilisof, A. M. R., & Bartholoni, G. B. (1991). Enzymatic modification of soy protein concentrates by fungal and bacterials protease. Journal of the American Oil Chemists Society, 68, 102-105.
Farah, I. (2010). Effect of degree of hydrolysis on physicochemical properties of cobia muscle hydrolysate, Unpublished report.
Gauthier, S. F., Paquin, P., Pouliot, Y., & Turgeon, S. (1993). Surface Activity and Related Functional Properties of Peptides Obtained from Whey Proteins. Journal of Dairy Science, 76(1), 321–328.
Gbogouri, G. A., Linder, M., Fanni, J., & Parmetier, M. (2004). Influence of hydrolysis on the functional properties of salmon byproduct hydrolysate. Journal of Food Science, 69, 615-622.
Haque, Z.U., & Mozaffar, Z. (1992). Casein hydrolysate II. Functional properties of peptides. Food Hydrocolloids, 5, 559-571.
He, X. Q., Cao, W. H., Zhao Z. K., & Zhang, C. H. (2013). Analysis of protein composition and antioxidant activity of hydrolysates from Paphia undulate. Journal of Food and Nutrition Research, 1(3), 30-36.
Hoyle, N. & Merritt, J. (1994). Quality of fish protein hydrolysate from herring (Clupea harengus). Food Science, 59(1), 76-79.
Jamdar, S. N., Rajalakshmi, V., Pednekar, M. D., Juan, F., Yardi, V., & Sharma, A. (2010). Influence of degree of hydrolysis on functional properties, antioxidant activity and ACE inhibitory activity of peanut protein hydrolysate. Food Chemistry, 121(1), 178–184.
Klompong, V., Benjakul., S., Kantachote, D., & Shahidi, F. (2007). Antioxidative activity and functional properties of protein hydrolysates of yellow stripe travelly (Selaroides leptolepsis) as influenced by degree of hydrolysis and enzyme types. Food Chemistry, 102, 1317-1327.
Kristinsson, H. G., & Rasco, B. A. (2000a). Fish protein hydrolysates: production, biochemical, and functional properties. Critical Review in Food Science and Nutrition, 40, 43-81.
Kristinsson, H. G., & Rasco, B. A. (2000b). Hydrolysis of salmon muscle proteins by an enzyme mixture extracted from Atlantic salmon pyloric caeca. Journal of Food Biochemistry, 24, 177-187.
Liceaga-Gesualdo, A. M., & Li-Chan, E. C. Y. (1999). Functional properties of fish protein hydrolysate from herring. Journal of Food Chemistry and Toxicology, 64, 1001-1004.
Liu, Y., Li, X., Chen, Z., Yu, J. and Wang, J. (2014). Characterization of structural and functional properties of fish protein hydrolysates from surimi processing by-products Food Chemistry, 151, 459-465.
Morr, V., German, B., Kinsella, J. E., Regenstein, J. M., Van Buren, J. P., & Kilara, A. (1985). A collaborative study to develop a standardized food protein solubility procedure. Journal of Food Science, 50, 1715–1718.
Pacheco-Aguilar, R., Mazorra-Manzano, M. A., & Ramirez-Suarez, J. C. (2008). Functional properties of fish protein hydrolysates from Pacific whiting (Merluccius productus) muscle produced by a commercial protease. Food Chemistry, 109, 782-789.
Puski, G. (1975). Modification of functional properties of soy proteins by proteolytic enzyme treatment. Cereal Chemistry, 52, 655.
Quaglia, G. B., & Orban, E. (1987). Influence of the degree of hydrolysis on the solubility of the protein hydrolysates from sardine (Sardina pilchardus). Journal of the Science of Food and Agriculture, 38(3), 271–276.
Rutherfurd, S. M. (2010). Methodology for determining degree of hydrolysis of protein in hydrolysates: A review. Journal of AOAC International, 93(5), 15-22.
Salwanee, S., Wan Aida, W., Mamot, S., Maskat, M.Y., & Ibrahim, S. (2013). Effects of enzyme concentration, temperature, pH and time on the degree of hydrolysis of protein extract from viscera of tuna (Euthynnus affinis) by using Alcalase. Sains Malaysiana, 42(3), 279-287.
Sathivel, S., Smiley, S., Prinyawiwatkul, W., & Becthel, P. J. (2005). Functional and nutritional properties of red salmon (Oncorhynchus nerka) enzymatic hydrolysates. Journal of Food Science, 7(6), 401-406.
Severin, S., & Xia, W. S. (2005). Enzymatic hydrolysis of whey protein by two different protease and their effect on the functional properties of resulting protein hydrolysates. Journal of Food Biochemistry, 30, 77-97.
Shahidi, F. (1994). Sea Food Processing By-Products. In: Seafoods: Chemistry, Processing Technology and Quality, F. Shahidi, J.R. Botta (eds.), Blackie Academic and Professional, London, UK. pp. 321–334.
Shahidi, F., Han, X. Q., & Synowicki, J. (1995). Production and characteristic of protein hydrolysates from capelin (Mallotus villosus). Food Chemistry, 53, 285-293.
Shu, G., Zhang, B., Zhang, Q., Wan, H., & Li, H. (2016). Effect of Temperature, pH, Enzyme to Substrate Ratio, Substrate Concentration and Time on the Antioxidative Activity of Hydrolysates from Goat Milk Casein by Alcalase. The Journal of Lucian Blaga, 20(2), 29-38.
Šližytė, R., Dauksas, E., Falch, E., Storro, I., & Rustad, T. (2005). Characteristics of protein fractions generated from hydrolysed cod (Gadus morhua) by-products. Process Biochemistry, 40(6), 2021–2033.
Souissi, N., Bougatef, A., Triki-Ellouz, Y. & Nasri, M. (2007). Biochemical and functional properties of sardinella (Sardinella aurita) by product hydrolysate. Food Technology and Biotechnology, 45, 187-194.
Tavano, O. L. (2013). Protein hydrolysis using proteases: An important tool for food biotechnology. Journal of Molecular Catalysis. B: Enzymatic, 90, 1–11.
Thiansilakul, Y., Benjakul, S., & Shahidi, F. (2007). Compositions, functional properties and antioxidative activity of protein hydrolysate prepared from round scad (Decapterus maruadsi). Food Chemistry, 103, 1385-1394.
Wasswa, J., Tang, J., Gu, X. H., & Yuan, X. Q. (2007). Influence of the extent of enzymatic hydrolysis on the functional properties of protein hydrolysate from grass carp skin. Food Chemistry, 104, 1698-1704.
Wilde, P. J., & Clark, D. C. (1996). Foam formation and stability. In: Methods for Testing Protein Functionality. Hall, G. M . (Ed.), p. 110-148. Blackie Academic and Professional, London, England.
Wong, C. (2012). Effect of degree of hydrolysis on physicochemical properties of blood cockle (Anadara granosa) hydrolysate by using enzymatic hydrolysis. Unpublished reports.
Zhou, H. M., Amza, T., Amadou, I., Kamara, M. T., & Zhu, K. X. (2011). Nutritional and functional characteristics of gingerbread plum (Neocarya macrophylla): An underutilized oilseed. Grasas Y Aceites, 62(3), 290-298.
